Part:BBa_K1897002

HasR Membrane receptor coding sequence

Usage and Biology

HasR is a 98.22 kDa membrane-bound receptor in the Has system, a heme acquisition system originally from the Serratia marcescens. The HasR protein is involved in binding with a heme-loaded holo-HasA hemophore to trigger downstream signalling events. The signal transduction fails to occur with exposure to only haem or the apo-HasA (Rossi et al., 2003). Furthermore, it has been shown that the N terminal of HasR is especially crucial for signal transduction (Biville et al., 2004). With the holo-hasA bound to HasR, it induces a conformational change in HasR that causes the activation of HasS, an anti-sigma factor. HasS then releases HasI (sigma factor) initially bound to it and HasI ultimately is able to trigger expression of genes under control of Has operon promoter.

Characterisation of this part is done in tandem with the full has operon (BBa_K1897007)

References

Biville, F., Cwerman, H., Létoffé, S., Rossi, M. S., Drouet, V., Ghigo, J. M., & Wandersman, C. (2004). Haemophore‐mediated signalling in Serratia marcescens: a new mode of regulation for an extra cytoplasmic function (ECF) sigma factor involved in haem acquisition. Molecular microbiology, 53(4), 1267-1277.

Rossi, M. S., Paquelin, A., Ghigo, J. M., & Wandersman, C. (2003). Haemophore‐mediated signal transduction across the bacterial cell envelope in Serratia marcescens: the inducer and the transported substrate are different molecules. Molecular microbiology, 48(6), 1467-1480.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 1866
  Illegal NotI site found at 2660
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 24
  Illegal NgoMIV site found at 28
  Illegal NgoMIV site found at 84
  Illegal NgoMIV site found at 202
  Illegal NgoMIV site found at 356
  Illegal NgoMIV site found at 421
  Illegal NgoMIV site found at 757
  
• 1000
  COMPATIBLE WITH RFC[1000]